• Bd Flow Cytometry
• Bd Facscanto User Manual

Advanced applications like this require a flow cytometer capable of high-speed analysis. The BD FACSCalibur™ system features a multi-parameter triggering system that allows a user to accelerate the analysis by electronically eliminating events from on-board processing. The BD FACSCanto II Flow Cytometer Reference Manual assumes you have a working knowledge of basic Microsoft® Windows® operation. If you are not familiar with the Windows operating system, refer to the documentation provided with your computer. BD FACSDiva software features specific to the BD FACSCanto cytometer within this manual. BD Biosciences recommends that first-time user’s of this instrument take advantage of operator training offered with the sale of every new instrument. The BD FACSCanto Flow Cytometer Reference Manual assumes you have a working knowledge of basic Microsoft. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible.

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DESCRIPTION

Bd Flow Cytometry

The BD Accuri™ C6 Plus offers simplified workflows and ready-to-use templates for performing a wide range of applications in flow cytometry. When combined with the portfolio of BD reagents and best-in-class service and support, it is an ideal solution for life science researchers performing routine assays in cellular analysis, while also reducing operating costs.
This new generation platform has several optimized enhancements to the internal hardware, fluidics, and software while maintaining the core elements of the prior platform such as the unique, non-pressurized, peristaltic pump system and compact exterior design. The ability to use BD CS&T RUO beads helps users with simplified QC methods, improving reliability of the experimental data.

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FEATURES

• Highly sensitive: MESF <75 (FITC) and <50 (PE)1 * Non-pressurized peristaltic pump system * Event rates up to 10,000 events per second * Supports sample collection from multiple sources, including * Uses laboratory-grade water as sheath fluid

  Flow cytometry within reach Easy to use, simple to maintain, and affordable, the BD Accuri™ C6 Plus personal flow cytometer brings cell analysis within reach for new applications and new flow cytometry users.

  Easy-to-Use Software with Automated QC and Application Templates BD Accuri C6 Plus software features an intuitive user interface. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Software options and instrument controls are clearly visible from the software’s tabbed interface

  Immunophenotyping and Cytokine Analysis Immunophenotyping was one of the first applications of flow cytometry, and for over 20 years, BD Life Sciences has actively supported groundbreaking immunological research with flow cytometry systems and reagents. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file.

  Rapid Analysis of Multiple Cellular Processes A personal flow cytometer in the lab provides many advantages for cell and cancer biology studies. When cells are ready for analysis or rare tumor samples arrive, it’s crucial to have a flow cytometer at hand, ready to go.

  Characterizing Stem Cells and Their Derivatives A primary challenge of stem cell research is to detect target cells within inherently heterogeneous cell cultures. The BD Accuri C6 Plus is ideal for this kind of research because of its ability to analyze the expression of multiple surface and/or intracellular markers at the single-cell level, quickly and simply.

  Microbial Analysis Across a Range of Applications In the diverse field of microbiology research, flow cytometry is a versatile and powerful technique for analyzing microorganisms including bacteria and yeast. The BD Accuri C6 Plus can provide rich data in a range of microbiological applications, such as measurement of gene expression, monitoring bacterial and yeast fermentations, recombinant protein production in bacteria, environmental research, food processing, and monitoring drinking water.

  Advanced Capabilities Expand Detection and Flexibility The BD Accuri C6 Plus is designed for compatibility with advanced polymer dyes such as BD Horizon Brilliant™ Blue 515 (BB515),which can help you detect low-density antigens and rare populations. Developed from patented technology and based on Nobel Prize-winning chemistry, BB515 is significantly brighter than FITC.

Bd Facscanto User Manual

SPECIFICATIONS

Additional Specifications

Sample Tubes:12 x 75-mm (or smaller)
Decade Dynamic Range: 7.2 (16 million channels of digital data)

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DESCRIPTION

The BD FACSCalibur, a fully integrated, multiparameter system, offers the performance and sensitivity needed to ensure the objective and reproducible results vital to laboratories.
Flow cytometry has evolved rapidly to incorporate the use of multiple fluorochromes to effectively identify and isolate, with optional sorting module, ** subset populations from a single sample. This maximizes the information gained from limited amounts of sample and makes assays more cost effective.
Its optical design, sample handling options, and sorting capabilities make the BD FACSCalibur™ system a valued tool throughout the world for routine cell analysis, assay development, verification, and isolation of cellular populations of interest.

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FEATURES

Optical path configurationThe BD FACSCalibur was the first system to provide standard multicolor analysis capabilities by incorporating an alignment free optical design, interbeam compensation, and dual-laser technology.

Dual laser design for multicolor analysisA dual-laser design provides the flexibility and sensitivity needed for multicolor analysis on the BD FACSCalibur system. Two lasers, an air-cooled argon laser and a red diode laser, are spatially separated for high sensitivity, minimal need for compensation, and flexibility in fluorochrome selection.

BD High Throughput Sampler (HTS) option**The BD™ High Throughput Sampler (HTS) option provides flexible, easy-to-use automation. For those who prefer to work with multiwell plates, BD Biosciences offers the first walkaway sample introduction device for 96- and 384-well plates. The HTS option allows users to rapidly acquire samples with the BD FACSCalibur™ flow cytometer in a microtiter plate. As a modular option for new or existing BD FACSCalibur flow cytometers, the HTS option is designed to speed through a variety of microtiter plates in less than 15 minutes* and support a wide range of applications including the BD™ Cytometric Bead Array (CBA) assay. The HTS option supports 96 U-, V-, and flat-bottom plates as well as 384-well microtiter plates.BD™ Plate Manager software allows you to create customized protocols with user-defined mixing, wash, and analysis parameters that can be reused.

BD FACS Loader OptionThe BD FACS™ Loader option available for the BD FACSCalibur system provides walkaway automation for routine applications. Featuring a removable 40-tube carousel, on-board mixing, and BD™ Worklist Manager software, the BD FACS Loader option offers superior flexibility to efficiently address the workflow demands of the busy laboratory.

Unique easy-to-use sorting technology (optional)**The BD FACSCalibur cell sorting option allows users to identify and isolate a population of interest. Sorting on the BD FACSCalibur occurs in a completely enclosed, aerosol-free environment for enhanced safety which is especially important when processing biohazardous samples. The sorting process is designed to be straightforward. After the population of interest is gated, simply click Acquire to begin the sort. After the sample is acquired and cells pass through the laser, a unique catcher tube mechanism moves in and out of the sample core stream at a rate of roughly 300 times per second to capture designated cells and direct them to a collection tube or to an optional cell concentrator module for further processing.

Data managementThe BD FACSCalibur™ system is appreciated for its user-friendly design, high sample throughput, improved workflow management through automation, and simple yet sophisticated software applications that enhance routine analyses.While performing any of a wide range of supported applications, the BD FACSCalibur system provides the tools to obtain results quickly, easily, and accurately.The ease of use of the BD FACSCalibur system is enhanced by the BD FACStation™ data management system, which has become one of the most popular, user-friendly data management systems in flow cytometry. The BD FACStation system automates many software functions and delivers high-performance acquisition and analysis tools for creating plots, gating, statistical analysis, and reporting.The BD FACStation workstation takes advantage of the intuitive Mac® environment by using simple pull-down menus and icons. It performs all the computing tasks required for fast, accurate results, including instrument setup, data collection, analysis, and management.

A comprehensive quality assurance systemBD Biosciences offers a complete family of products designed to ensure the highest quality clinical results from the BD FACSCalibur system. BD FACSComp™ software, in conjunction with BD Calibrite™ beads, provides a comprehensive system to set up the instrument, assess sensitivity, and set compensation for immunofluorescence applications. For clinical applications, BD Biosciences offers a wide variety of cellular controls to verify performance for immunophenotyping, reticulocyte analysis, stem cell enumeration, and rWBC applications.

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Additional Specifications

Signal Processing
Workstation resolution: 1,024 channels on all parameters.
Dynamic range: Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option), providing four log decade range.
Fluorescence compensation networks: Compensation for fluorescence spectral overlap between FL1 and FL2, between FL2 and FL3, and between FL3 and FL4 channels (with FL4 option).
Pulse processing: Width and area measurements for discriminating doublets; available for all fluorescence parameters.
Time: Time available correlated to any parameter for kinetic experiments or other applications.
Sample Loading Specifications
Sample delivery: Tube-lifter design with multiple sensors that verify rack identification and tube position with optional BD FACS™ Loader.
Rack capacity: 40 (12 x 75-mm) tubes per rack.
Rack support: Up to 16 racks per BD FACS Loader.
Data entry: Sample information, reagent panels, and rack information can be defined for up to 640 tubes (40 tubes x 16 racks) at a time.
Loader manager: Automated control through BD™ Worklist Manager software.
Barcode scanner (optional): Automates data entry for Codabar, Code 39, Interleaved 2 of 5, Code 2 of 5, and Code 128.
Sorting Specifications* (for sorting option)
Sorting purity*: >95%
Capture rate*: 300 cells/second
Sort modes*: Three modes (all aerosol free): single cell, exclusion, and recovery.
Recovery*: Depending upon sample and sorting conditions, >50%.
Sterile sorting*: System design allows for aerosol-free sterile sorting.
Data Management System
Workstation: BD FACStation™ Mac Pro computer Processor 2.8-GHz Quad-Core Intel® Xeon® processor
Memory: 2 GB RAM
Level 2 cache: 4 MB per processor
Data storage: 320-GB 7200-rpm Serial ATA, 3Gb/s
Networking: On-board Ethernet, built-in AppleTalk® Networking, and Apple® File Sharing
Optical drive: 16x SuperDrive
Monitor: 17-inch LCD monitor (optional)
Data file structure: Flow Cytometry Standard (FCS) 2.0/3.0 ASCII results file for data export
Installation Requirements
Power**: US: 120 VAC ±10%, 50/60 Hz ±2 Hz,
Current: 20 amp maximum outside US. External transformer needed for 100 VAC ±10%, 50/60 Hz ±2 Hz, and 220/240 VAC ±10%, 50/60 Hz ±2 Hz.
Water supply: None required
Air supply: None required
Heat dissipation: BD FACSCalibur system with all options 6,500 BTU/h
Operating Environment
Temperature: 16–29°C (60–85°F)
Humidity: 10% to 90% relative non-condensing
Air filtering: No excessive dust and smoke
Lighting: Optics and detectors shielded from room lighting
Size: